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linescan function  (MetaMorph Inc)

 
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    Structured Review

    MetaMorph Inc linescan function
    Linescan Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/linescan+function+of/linescan+function/pm38201668-100-13-15
    Average 90 stars, based on 1 article reviews
    linescan function - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Atypical Protein Kinase C and Par3 Are Required for Proteoglycan-Induced Axon Growth Inhibition
    Article Snippet: For Par3, in each acquired cell image, a plane containing the brightest Par3 signal in the axonal compartment was selected, a line was traced along the axon, and its average pixel intensity was measured with the linescan function of MetaMorph.

    Article Title: Capping protein is dispensable for polarized actin network growth and actin-based motility
    Article Snippet: The linescan function of MetaMorph was used on the combined images, drawing a line from the center of the bead towards the outside.

    Article Title: Xenopus Meiotic Microtubule-Associated Interactome
    Article Snippet: To measure the relative intensities of GFP-tagged proteins on the spindles assembled in egg extracts we used the linescan function of Metamorph on 12-bit images taken under identical conditions.

    Article Title: Action potential initiation and propagation in CA3 pyramidal axons.
    Article Snippet: Meeks JP, Mennerick S. Action potential initiation and propagation in CA3 pyramidal axons.. J Neurophysiol 97: 3460–3472, 2007.. First published February 21, 2007; doi:10.1152/jn.01288.2006.

    Article Title: The role of the proteins Kar9 and Myo2 in orienting the mitotic spindle of budding yeast.
    Article Snippet: The ‘Linescan’ function of Metamorph provided numerical values and a graph representing the grayscale pixel values along a linear region for a single image plane from a time-lapse series.

    Fluorescence:

    Article Title: Mast cells form antibody-dependent degranulatory synapse for dedicated secretion and defence.
    Article Snippet: .. Av.SRho fluorescence is expressed as percentage of IFI measured in each region. (e) Analysis of chymase, tryptase and Av.SRho fluorescence signal localization using the Linescan function of MetaMorph software. ..

    Staining:

    Article Title: Activation of the ancestral polarity regulator protein kinase C zeta at the immunological synapse drives polarization of Th cell secretory machinery toward APCs.
    Article Snippet: .. B, Intensity of p-PKCz staining was measured using the Linescan function of MetaMorph as in Fig. 1 (see scheme). ..

    Activation Assay:

    Article Title: A wave of free cytosolic calcium traverses zebrafish eggs on activation.
    Article Snippet: .. Our method of analyzing activation wave velocities using the Linescan function of Metamorph (see Materials and Methods) result in “axial velocities”; i.e., it assumes that the wave passes directly through the egg along the AP to the VP axis. ..



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    GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.
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    GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) <t>Linescan</t> measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.
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    Image Search Results


    GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) Linescan measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Locally-Induced CaMKII Translocation Requires Nucleotide Binding

    doi: 10.3389/fnsyn.2020.00004

    Figure Lengend Snippet: GFP-calcium-calmodulin-dependent protein kinase (CaMKII) expression and translocation after stimulation with 100 μM glutamate with 10 μM glycine via pipette. (A) GFP-CaMKII expression prior to stimulation. (B) DIC image of the same neuron with measured dendrites highlighted in red (right) and blue (left) and an arrow indicating the position of the micropipette. Scale bar = 50 μm. (C) Time-lapse subtraction images (current fluorescence level minus initial fluorescence level; increases in fluorescence appear whiter while decreases are darker/black). Hollow white arrows indicate dendrite locations that show increased relative fluorescence over time, solid white arrow indicates the location where relative fluorescence level decreases. (D) Linescan measures of the same neuron shown in (C) indicating fluorescence levels for respective dendrites, red (top) and blue (bottom), starting proximal to the cell body. Brackets denote regions that correspond to boxes shown in (A,B) . Black linescan trace indicates fluorescence levels measured at first post-stimulation image capture for comparison. Arrowhead indicates the location of the stimulation pipette.

    Article Snippet: The density of GFP-CaMKII puncta was calculated by dividing the number of identified puncta by the length of the dendrite measured using the MetaMorph linescan function yielding a value in puncta/μm.

    Techniques: Expressing, Translocation Assay, Transferring, Fluorescence